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Kinetic Behaviour of the Foam Films from Aqueous Solutions of Bovine Serum Albumin and n-dodecyl-beta-D-maltoside // Кинетично поведение на пенни филми от водни разтвори на говежди серумен албумин и n-додецил-бета-D-малтозид
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Experimental study of the kinetic behavior and the equilibrium state of foam films formed from mixed aqueous solutions of bovine serum albumin and n-dodecyl-beta-D-maltoside; using data from it for the evaluation of co-adsorption of BSA on the film phase boundaries and utterance hypothesis of the composition of the films. The aim of the present study was implemented through the following tasks: • to study the interaction between albumin and n-dodecyl-beta-D-maltoside in mixed solutions with two independent methods; • to test the surface activity of the individual solution of BSA, C12G2 and their mixed solutions with different molar ratio between the components in them; • to determine the thickness and composition of the adsorption layers formed from solutions of BSA, C12G2 or their mixed solutions, by atomic force microscopy; • to register the evolutions (from formation of the film to its equilibrium state) of the foam films from solutions of BSA, C12G2 or their mixed solutions by a modified interferometric method; from them to distinguish the stages through which the film goes from its formation to the equilibrium state; to derive from them an qualitative information about the films kinetic behavior and to determine the films equilibrium parameters. • to study the kinetic behavior of the foam films from solutions of BSA, C12G2 or mixed their solutions, by determining: the velocity of their thinning (alpha)in different areas in the film; the thickness in same areas at the time of the film critical state and the characteristic times tau01 and tau12; • to evaluate the co-adsorption of BSA in foam film of a mixed solution of BSA and C12G2 with molar ratio of the components - 1:100; • to suggest the hypothesis for the composition of the films from the mixed solutions of BSA and C12G2 on the base of data: (i) for the interaction between BSA and C12G2 molecules in the solution and on the films surfaces; (ii) for the surface tension of the solutions; (iii) from the AFM images; (iv) for the viscosity in the solutions and in the films.